Ku70 (E-5): sc-17789 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80).

Ku70 is a protein that, in humans, is encoded by the XRCC6 gene.

The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. 2001-03-01 Invitrogen Anti-Ku70 Recombinant Monoclonal (JM61-31), Catalog # MA5-32645. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human samples.

ku70 tert : gl1; ku70; tert: images; None available : phenotypes ; Surovtseva YV, Shakirov EV, Vespa L, Osbun N, Song X, Shippen DE(2007) In contrast to the ku70 mutant, which undergo telomerase-dependent expansion to more than twice the normal length in a single generation, the ku70 tert double mutant displays accelerated telomere shortening and a precocious onset of genome stability. Plasmid pEGFP-C1-FLAG-Ku70 from Dr. Steve Jackson's lab contains the insert Ku70 and is published in J Cell Biol. 2013 Jul 29. This plasmid is available through Addgene. Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-27538. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples.

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Recognition of DNA double‐strand breaks during non‐homologous end joining is carried out by the Ku70–Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) to the lesion. In this study, CBP was found to positively regulate the expression of Ku70, and both CBP and Ku70 were found to negatively regulate the expression of NOX2, therefore, mitigating the intracellular The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins.

Because Ku70 sequesters Bax from mitochondria, cells overexpressing Bax alone are more susceptible to apoptosis than are cells overexpressing both Bax and Ku70. In the presence or absence of Ku70 overexpression, 293T cells grown in serum from CR animals were less susceptible to Bax-mediated apoptosis as compared to cells grown in media with AL serum ( Fig. 1D ).

Significantly, LPS-induced NFκB activation was inhibited by Ku70 plus Ku80 double knockdown or DKO. It was however enhanced with Ku70 and Ku80 overexpression. The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway.

In addition to NHEJ, Ku70/80 also plays an important role in multiple biological processes including telomere maintenance [], HIV replication [] and suppression of apoptosis []. Ku70 and Ku80 form a pseudosymmetrical heterodimer with a preformed ring (also called DNA aperture) responsible for sequence‐independent DNA binding.

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Validated in WB, ICC/IF, IHC-P, IP, PLA. Tested in Human, Mouse. Cited in 11 reference(s). Independently reviewed in 1 Unconjugated Whole IgG Rabbit anti-Ku70 Antibody, Affinity Purified suitable for WB, IP applications. Visit Bethyl.com for all your antibody needs. Ku70 and Ku80 participate in LPS-induced pro-inflammatory cytokines production in human macrophages and monocytes. Hong Sun 1, *, , Quan Li X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 6. THYROID AUTOANTIGEN, 70-KD; G22P1 Ku ANTIGEN, 70-KD SUBUNIT; Ku70 The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA Nov 19, 2019 Moreover, chromatin recruitment of key NHEJ proteins, including, Ku70, Ku80, DNA-PKcs and XLF was diminished in autophagy-deficient cells.
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Ku70 Antibody (PA5-27538) in WB Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5). Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination. loss of expression correlates with decreased survival in gall bladder malignancies patients SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax. Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair.

Validated in WB, ICC/IF, IHC-P, IP, PLA. Tested in Human, Mouse. Cited in 11 reference(s).
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Unconjugated Whole IgG Rabbit anti-Ku70 Antibody, Affinity Purified suitable for WB, IP applications. Visit Bethyl.com for all your antibody needs.

In this study, CBP was found to positively regulate the expression of Ku70, and both CBP and Ku70 were found to negatively regulate the expression of NOX2, therefore, mitigating the intracellular The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex.

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Immunogen Synthetic peptide corresponding to Human Ku70 aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin. (Peptide available as ab92421)

-Ku80ヘテロ二量体と相互作用することにより非相同末端結合によるDNA 2 本鎖切断の修復を促進し，非相同末端結合にかかわるタンパク質をDNA 2本鎖 ku７０/ku８０のヘテロダイマーにDNA―PKcs が結合し、DNA―PKcs が活性化します。その後、artemis によってDNA 切断端を修飾し、ligaxe IV とXRCC４ Feb 1, 2013 Ku70 was originally described as an autoantigen, but it also functions as a DNA repair protein in the nucleus and as an antiapoptotic protein by Apr 26, 2009 for its apoptotic one, Ku70 acts as part of a heterodimer with an approximately 80 kDa protein, Ku80. In contrast to the signifi- cant amount of Feb 28, 2013 Analysis of Ku70−/− pancreatic β-cells revealed an accumulation of DNA damage and activation of p53-dependent cellular senescence similar Ku70 is a protein that, in humans, is encoded by the XRCC6 gene. Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance. It is a heterodimeric protein made up of Ku70 and Ku80.

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2. Results 2.1. The Enhanced Cyan Fluorescent Protein (ECFP)-Ku70/EYFP-Ku80 FRET Pair The Ku70/80 heterodimer binds to DNA ends as a starting point for NHEJ complex assembly and juxtaposition Ku70 (E-5): sc-17789 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80). In addition to NHEJ, Ku70/80 also plays an important role in multiple biological processes including telomere maintenance [], HIV replication [] and suppression of apoptosis []. Ku70 and Ku80 form a pseudosymmetrical heterodimer with a preformed ring (also called DNA aperture) responsible for sequence‐independent DNA binding. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS.

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Recognition of DNA double‐strand breaks during non‐homologous end joining is carried out by the Ku70–Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) to the lesion. In this study, CBP was found to positively regulate the expression of Ku70, and both CBP and Ku70 were found to negatively regulate the expression of NOX2, therefore, mitigating the intracellular The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins.